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By Philip S. Mellor, Matthew Baylis, Christopher Hamblin, Charles H. Calisher, Peter P.C. Mertens

African horse disease virus is a double-stranded RNA virus which factors a non-contagious, infectious arthropod-borne illness of equines and infrequently canines. 9 certain, the world over acknowledged serotypes of the virus have up to now been pointed out. This ebook is predicated upon the findings of 2 programmes funded by way of the ecu fee. will probably be of price not just to the professional study staff but additionally to veterinary staff facing keep an eye on and to legislators trying to advertise secure foreign circulation of equines. the themes coated comprise cutting-edge discussions on diagnostics, vaccines, molecular biology, vector reports, and epidemiology.

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7 were considered positive. Serotype specific antibodies against AHSV 4 and 5 were determined by virus neutralisation (VN) tests. Assays were performed in micro titre plates using BHK-21 tissue culture cells [10]. Antibody titres were expressed as reciprocalloglO of the final dilution of serum in the serum/virus mixtures at the 50% end-point [12]. Results Study 1 No clinical signs were observed nor was pyrexia recorded in any of the donkeys (D1-D4) following inoculation of AHSV 4 into three different sites.

Oxford University Press, Oxford, pp 460-475 5. Finney DJ (1964) Statistical methods in biological assay, 2nd ed. Griffin, London, pp 524-553 6. Goldsmit L, Barzilai E, Tadmor A (1975) The comparative sensitivity of sheep and chicken embryos to bluetongue virus and observations on viraemia in experimentally infected sheep. Aust Vet J 151: 190-196 7. Hamblin C, Graham SD, Anderson EC, Crowther JR (1990) A competitive ELISA for the detection of group-specific antibodies to African horse sickness virus.

Hamblin et al. regions of the Middle East. However, the virus does make occasional excursions outside the endemic regions, the most recent being the un-precedented epizootic involving Spain, Portugal and Morocco, which occurred between 1987 and 1991 [14]. Despite repeated and widespread vaccination of equidae, African horse sickness virus (AHSV) persisted in one or more of these countries for 5 years. The role of the donkey in the epidemiology of AHS during an epizootic is poorly defined. Previous workers [2, 3] have reported some clinical disease and deaths of donkeys during epizootics of AHS in the Middle East.

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